This doesn't seem too far fetched. All you have to do is add the excel file using Datasource step(if more than one file, use more Datasource steps). You will have to create a property against each column in the Datasource. Now that you have a property for each column, you can easily assert against a particular value,once you have a valid response go to the outline view>right click on the node that you want to validate against>click on add assertion>For content. In the expected value below Remove what in there already> right click>Click on Get Date> and point to the property you want to validate against within the Datasource Step.
More info on Point and click testing(Get Data):
http://www.soapui.org/Working-with-soap ... sting.htmlMore on Property expansion:
http://www.soapui.org/Scripting-Propert ... nsion.htmlLet me know if you need anything else.
Regards,
Temil