Hi you can overcome that situation by enabling anonymous access on your website.
After that you need to do the following so that you dont get the 401 unauthorized error..
soapUI doesn’t seem to work directly with NTLM authentication, but you can use a proxy such as Burp Suite to do the auth for you.
Download Burp Suite from
http://www.portswigger.net/burp/downloadfree.html and install it with default settings
On Burp’s “Proxy : Intercept” tab, click the button to turn intercept off.
On Burp’s “Proxy : Options” tab, make sure it’s set to an unused port, the default is 8081
On Burp’s “Options” tab, tick “do www authentication” and add a setting for the server you wish to hit. Also tick “prompt for credentials on authentication failure”
Switch to Burp’s “Proxy : History” tab so you can see requests going through.
In SoapUI, choose File > Preferences, then select “Proxy Settings”. Enter Host “localhost” and port “8081″.
Use SoapUI as normal. It will send requests through Burp Proxy, which will do the NTLM authentication for you.