Hi, CollinRichman.
JMS Request test step has send and receive destinations, so yes, you can configure sending a message to an input queue and receiving a response from an output queue. Also it's possible to leave either of them blank, in which case JMS Request step will only send a message without waiting for a response, or immediately start listening for a message without sending anything. Read more here: http://readyapi.smartbear.com/structure/steps/request/jms/add (section New Endpoint shows the dialog to configure JMS endpoint).
